Active Research Using GrandFusion®

Reduced neuroinflammation and improved functional recovery after traumatic brain injury by diet supplementation in mice  (PENDING)

The effect of diet on improved endurance in male C57BL/6 mice

Abstract

In the current study, the impact of a diet enriched in fruits and vegetables (GrandFusion®) on exercise endurance was examined in a mouse model.  GrandFusion (GF) diets increased mitochondrial DNA, enzyme activity and stimulated mitochondrial mRNA synthesis in vivo.  GF diets increased both mRNA expression of factors involved in mitochondrial biogenesis, peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1a), mitochondrial transcription factor A (Tfam), estrogen-related receptor alpha (ERRa), nuclear respiratory factor 1 (NRF-1), cytochrome c oxidase IV (COXIV) and ATP synthase (ATPsyn).  Mice treated with GF diets showed an increase in running endurance, rotarod perseverance and grip strength when compared to regular diet controls.  In addition, GF diets increased the protein expression of phosphorylated AMP-activated protein kinase (AMPK), sirtuin 1 (SIRT1), PGC-1a and peroxisome proliferator-activated receptor delta (PPAR-d) over exercise related changes.  Finally, GF reduced the expression of phosphorylated ribosomal protein S6 kinase 1 (p-S6K1) which demonstrated a decreased in autophagy.  These results demonstrate that GF diets enhance exercise endurance mediated via mitochondrial biogenesis and function.

Daily supplementation with GrandFusion® improves memory and learning in aged rats

Abstract

Studies have shown that supplementation with extracts from various sources, including fruits and vegetables reverse the age-related changes in movement and cognition. We hypothesized that these beneficial effects result from the presence of anti-oxidants and anti-inflammatory compounds in the fruits and vegetables that contribute to reduced oxidative stress, inflammation and cell death while potentially enhancing neurogenesis. The present study was performed to determine the impact of supplementation with GrandFusion®(GF) to aged Fisher 344 rats for 4 months to determine the impact on attenuation or reversal of the age-related deficits. When the aged rats consumed a diet enriched with the extracts the results showed an improved motor performance, and enhanced cognitive functions. In addition, the rats showed reduced oxidative stress and inflammation, and enhanced neurogenesis, Nrf2 and anti-oxidant expression. The effect of GF extracts on the augmentation of memory and learning is significant and may function through the modulation of antioxidant enzymes, signaling pathways and additional mechanisms to improve the aging process. These studies further support the recommendation of USDA for the consumption of fruits and vegetables to improve healthy aging.

Dietary supplementation of GrandFusion® mitigates cerebral ischemia-induced neuronal damage and attenuates inflammation.

Abstract

OBJECTIVES:

Dietary supplementation of fruits and vegetables has been the main stay for nutritional benefit and overall well-being. GrandFusion(®) is a nutritional supplement that contains the natural nutrients from whole fruits and vegetables that include complex nutrients and phytonutrients that contain anti-oxidant, anti-inflammatory, and neuroprotective properties.

METHODS:

In this study, C57BL/6 mice were fed a diet supplemented with GrandFusion(®) for 2 months prior to 1 hour of ischemia induced by occlusion of the middle cerebral artery (MCAo) followed by various times of reperfusion. Mice were subjected to MCAo for 1 hour and then at various times following reperfusion, animals were assessed for behavioral outcomes (open field testing, rotarod, and adhesive test removal), and infarct volumes (cresyl violet and triphenyltetrazolium chloride). In addition, to determine the potential mechanisms associated with treatment, the brain tissue was examined for changes in oxidative stress and inflammatory markers.

RESULTS:

The GrandFusion(®) diet was able to show a significant protection from infarct damage in the brain and an improvement in neurological outcomes. The diet did not alter heart rate, blood pressure, pO2, pCO2, or pH. In addition, the diet mitigated inflammation by reducing microglial and astrocytic activation following ischemia and reperfusion and limiting oxidative stress.

DISCUSSION:

The study demonstrates the neuroprotective effect of a diet rich in fruits and vegetables that contain anti-oxidant and anti-inflammatory against the impact of cerebral ischemia and reperfusion injury.

CURRICULUM VITAE
Mark Stephen Kindy, Ph.D.